اثرات عصاره‌ی الکلی مریم گلی (Salvia Officinalis L.) بر پارامترهای بیوشیمیایی خون در موش‌های صحرایی نر

Background and Objective: salvia officinalis L. consists of, glycosides, flavonoids, and anthocyanins. Due to the increasing use of medicinal plants in treatment of diseases and their side effects on various organs, this study was conducted to evaluate the effect of salvia officinalis on biochemical blood parameters in male rats. Materials and Methods: In this experimental study, 24 male Wistar rats were randomly assigned to four groups of 6 animals each. The control group received normal saline and treatment groups received 75, 150 and 300 mg/kg body weight of salvia extract for 14 days. Body weight was measured in days 0, 7, and 14. At the end of the experiment, serum levels of liver function enzymes such as AST and ALT, total proteins, albumin, creatinine, cholesterol and triglyceride were assessed. Results were analyzed using one- way ANOVA and Tukey test. Results: According to the results, plasma concentrations of protein, albumin and creatinine showed a significant increase (P<0.05) but, a significant decrease was observed in liver enzymes. No significant changes were observed in serum cholesterol, triglyceride levels and body weight in the control and salvia groups (P>0.05). Conclusion: The results indicated that Salvia Officinalis probably contains flavonoidic components with antioxidant effect leading to the increase in albumin and decrease in liver enzymes. References 1- Katzung B, Masters S, Trevor A. Basic & clinical pharmacology. Newyork: Mc Graw-Hill Medical. 2011. 2- Glisic SB, Ristic M, Skala D, Ivanovic J. Extraction of sage (salvia officinalis L.) by supercritical Co2: kinetic data, chemical composition and selectivity of diterpenes. J Superitica Fluids. 2010 55(1): 62-70. 3- Oboh G, Henle T. Antioxidant and inhibitory effects of aqueous extracts of salvia officinalis leaves on pro-oxidant-induced lipid peroxidation in brain and liver in vitro. J Med Food. 2009 12(1): 77-84. 4- Wang M, Shao Y, Li J, et al. Antioxidative phenolic glycosides from sage (Salvia officinalis). J Nat Prod. 1999 62: 454-6. 5- Baricevic D, Sosa S, Della Loggia R, et al. Topical anti in flammatory activity of Salvia officinalisl leaves. J. Ethnopharmacol. 2001 75: 125-32. 6- Weiss RF, Fintelman Volker. Herbal Medicine Germany: Thieme Verlag. 2001. 7- Orhan I, Kartal M, Naz Q, et al. Antioxidant and anticholinesterase evaluation of selected turkish salvia species. Food Chemistry. 2007: 103: 1247-54. 8- Tepe B, Eminagaoglu O, Akpulat H, Aydin E. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. Verticillata and S. verticillata (L.) subsp. Amasiaca (Freyn & Bornm.) Bornm Food Chemistry. 2007 100: 985-9. 9- Abd-Elmageed MAM, Hussein BA. Cytotoxicity and antimicrobial activity of Salvia officinalis L. flowers. Sudan JMS. 2008 3(2): 127-33. 10- Krinke GJ. History, strains and models. The laboratory rat Hand book of Experimental Animals. UK: Academic Press. 2000. 11- Zhong QW, Zhang HX, Russeell JC, Hulver M, Cefalu WT. Chromium picolinate enhances skeletal muscle cellular- Resistant JCR: LA-cp Rats. J. Nutr. 2006 136: 415-20. 12- Zhanq H, Chen L, Zhanq J, et al. Effect of Salvia miltiorrhizae on apoptosis and NF-kappaB p65 expression in the liver of rats with severe acute pancreatitis or obstructive jaundice. J. Gastroenterol Hepatol. 2009 24(5): 841-52. 13- Zargari A. Pharmaceutical plants (persian). Tehran: Tehran University Press 1997. 14- Sahin F, Gulluce M, Daferera D, et al. Biological activities of the essential oils and methanol extract of Origanum Vulgare in the Eastern Anatolia region of Turkey. Food Control. 2004 15 (7): 549-57. 15- Shishenian B, Farzin S. Medical hematology.Tehran: Daneshjo Press 1999. 16- Thomas L. Clinical laboratory diagnostics. Frank Furt: TH-Books Verlagsgesell schaft. 1998. 17- Mirzaee A, Hakimi MH, Sadeghi H. Total antioxidant activity and phenolic content of Dorema aucheri: Iran J Biocheme Mol Biol. 2005 1: 11. 18- Pizzale L, Bortolomeazzi R, Vichi S, Uberegger E, Conte LS. Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanumonites and O indercedens) extracts related to their phenolic compound content. J Sci Food Agric. 2002 82: 1645-51. 19- Lu Y, Foo L.Y. Flavonoid and phenol glycosides from Salvia Officinalis. Photochemistry. 2000 55: 263-7. 20- Esmaeili MA, Sombol A, Kanani M, Sadeghi H, Karimian Pour N. Evaluation of the effect of Salvia Sahandica on tissue damages induced by alcohol in oxidative stress conditions in the rat: effect on liver and kidney oxidative parameters. Pharmalogical Sciences. 2008 15: 315-22. 21- Yurtseven S, Cetin M, Sengul T, Sogut B. Effect of sage extract (Salvia Officinalis) on growth performance, blood parameters, oxidative stress and DNA damage in partridges. South African Journal of Animal Science. 2008 38 (2): 145-152. 22- Cassarett Douls. Toxicology: The basic science of poison. 6th ed. Newyork: McGraw-Hill 2001. 23- Cristovao F, Patricia C, Paula B, Rosa M, Manuel F, Cristina p. Water and methanolic extracts of Salvia Officinalis protect HepG2 cells from t-BHP induced oxidative damage. Chemico-Biological Interactions. 2007 167: 107-15. 24- Khakpour Sh, Khosravi M, Tajaddod G, et al. Evaluation of the effect of Salvia officinalis extract on liver in the rat. Tehran: Tehran University Press 2013. 25- Soochan D, Keough V, Wanless I, Molinari M. Intra and extra-hepatic cystadenoma of the biliary duct. Review of literature and radiological and pathological characteristics of a very rare case. BMJ Case Rep. 2012 25: 110-6. 26- Hall JE. Guyton and Hall textbook of medical physiology. 12th ed. New York: Saunders 2010.